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Hence, they were trained to be aggressive.

We established a reproducible ablation site just after the last cluster of L1 neural cell bodies Figure 1b , arrow , such that the projections of all neurons along the L1 margin were axotomized. For 1d animals compared to the earlier timepoint of 6h, the response was generally similar, while the 7d and 14d responses showed more widespread loss of GFP extending from the injury site along the wing margin.

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Initial studies piloting protocols and defining the responses were done on many animals. We also examined the severed axonal ends for signs of fragmentation indicative of Wallerian degeneration.

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